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. 2006 Sep 27;80(24):11946–11959. doi: 10.1128/JVI.01722-06

FIG. 3.

FIG. 3.

Growth of v-cyclin mutants is similar to that of wild-type (WT) γHV68 in vitro. NIH 3T12 fibroblasts were infected with 10 PFU (A) or 0.01 PFU (B) per cell of wild-type γHV68 (closed diamonds), v-cyc[STOP] (closed squares), v-cyc[STOPMR] (open squares), v-cyc[K104E] (closed circles), v-cyc[K104MR] (open circles), v-cyc[E133V] (closed triangles), or v-cyc[E133MR] (open triangles). Inocula were removed, and samples were washed with PBS and repleted with fresh medium 1 h postinfection for determinations of single-step growth (A) or left for multistep growth analysis (B). Samples were harvested at the indicated times, and viral titers were determined by plaque assay. (C) Growth of wild-type γHV68 and v-cyclin mutants in lung epithelial cells in vitro. LA-4 lung epithelial cells were plated in low-serum medium, allowed to grow to contact inhibition, and rested for 72 h. Cells were then infected with 0.05 PFU wild-type γHV68 (closed diamonds), v-cyc[STOP] (closed squares), v-cyc[K104E] (closed circles), or v-cyc[E133V] (closed triangles) per cell in low-serum medium. Samples were harvested at the indicated times, and viral titers were determined by plaque assay. All data (A to C) are representative of two independent experiments, with titers of each sample determined in duplicate.