TABLE 1.
Summary of acute virus replication and reactivation from latency
| Virus | Mean log10 titer± SEMa
|
Frequency of ex vivo reactivationb
|
||||||
|---|---|---|---|---|---|---|---|---|
| Following i.n. administration in lung
|
Following i.p. administration in spleen
|
Day 16 splenocytes | Day 42
|
|||||
| Day 4 | Day 9 | Day 4 | Day 9 | Splenocytes | Stimulated splenocytes | PECs | ||
| Wild-type γHV68 | 3.63 ± 0.20 | 3.71 ± 0.33 | 2.40 ± 0.13 | 3.80 ± 0.07 | 1/2,000 | LDc | 1/109,000d | 1/9,500 |
| STOP | 2.37 ± 0.19 | 2.61 ± 0.25 | 2.42 ± 0.11 | 3.08 ± 0.15 | 1/85,000 | LD | 1/250,000d | 1/120,000d |
| STOPMR | 3.72 ± 0.24 | 4.33 ± 0.33 | 2.75 ± 0.15 | 3.51 ± 0.11 | 1/2,000 | LD | 1/87,000 | NDe |
| K104E | 2.01 ± 0.13 | 2.36 ± 0.14 | 2.27 ± 0.12 | 3.45 ± 0.08 | 1/80,000 | LD | 1/105,000d | 1/14,700 |
| K104MR | 3.12 ± 0.23 | 3.72 ± 0.25 | 2.86 ± 0.09 | 3.64 ± 0.09 | 1/4,000 | LD | 1/90,000 | ND |
| E133V | 2.03 ± 0.16 | 2.31 ± 0.25 | 2.68 ± 0.18 | 3.38 ± 0.12 | 1/7,400 | 1/260,000d | 1/61,000 | 1/13,800 |
| E133MR | 3.24 ± 0.24 | 3.76 ± 0.30 | 3.21 ± 0.37 | 3.59 ± 0.20 | 1/1,800 | LD | 1/94,000 | ND |
a
i.n., intranasal; i.p., intraperitoneal.
b
Determined from the mean of three to six independent experiments with splenocytes or PECs pooled from four to five mice per experimental group.
c
LD, below the limit of detection of the assay (cannot be reliably extrapolated).
d
Value extrapolated from the best-fit curve of nonlinear regression.
e
ND, not determined.