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. 2006 Sep 27;80(24):12357–12366. doi: 10.1128/JVI.01207-06

FIG. 2.

FIG. 2.

Encapsidation of sgRNA3a. (A) BMV RNA was extracted from a purified virus preparation either by two cycles of PEG precipitation or by PEG precipitation and additional ultracentrifugation at 100,000 × g. The extracted RNA was separated in an agarose denaturing gel and stained with ethidium bromide. Lane 1, nt 1 to 1221 transcribed RNA3 size standard; lanes 2 and 3, virion RNA after two PEG precipitations at 2 and 10 days postinoculation; lanes 4 and 5, virion RNA after ultracentrifugation at 2 and 10 days postinfection. The encapsidated sgRNA3a and other BMV RNAs are indicated by arrows. (B) Northern blot analysis of BMV RNA extracted from a virus preparation 10 days postinoculation by using an RNA3-specific probe as for Fig. 1. Lanes 1 and 2, BMV RNA from a viral preparation after two PEG precipitations and after additional ultracentrifugation, respectively; lane 3, nt 1 to 1221 transcribed sgRNA3a size standard.