TABLE 1.
Heterogeneity of the 3′ oligo(A) tail in total RNA and in sgRNA3a virion moleculesa
| Source and no. of clone | 3′ Terminal sequence (no. of A residues) | % Total RNA | ||
|---|---|---|---|---|
| sgRNA3a from total RNA
|
||||
| 1 | poly(A) (13) | 10.0 | ||
| 6 | poly(A) (17) | 60.0 | ||
| 2 | poly(A) (18) | 20.0 | ||
| 1 | poly(A) (19) | 10.0 | ||
| Encapsidated sgRNA3a
|
||||
| 1 | poly(A) (14) | 5.5 | ||
| 6 | poly(A) (13) | 33.0 | ||
| 1 | poly(A) (9) | 5.5 | ||
| 2 | poly(A) (8) | 11.0 | ||
| 3 | poly(A) (7) | 17.0 | ||
| 1 | poly(A) (5) | 5.5 | ||
| 3 | poly(A) (3) | 17.0 | ||
| 1 | poly(A) (2) | 5.0 | ||
a
The number of poly(A) residues was determined by sequencing of RT-PCR-generated cDNA clones from either total RNA or virion BMV RNA preparations, as described in Materials and Methods.