TABLE 2.
Distribution of termination sites during in vitro copying of minus-strand RNA3 templates with the BMV replicase enzymea
| Termination site (wt RNA3)b | Site within the INT sequencec | Number of clones | Frequency of termination (%) |
|---|---|---|---|
| 1204 | Oligo(U) (3rd U) | 2 | 15.4 |
| 1216 | Oligo(U) (13th U) | 1 | 7.7 |
| 1208 | Oligo(U) (7th U) | 1 | 7.7 |
| 1203 | Oligo(U) (1st U) | 1 | 7.7 |
| 1210 | Oligo(U) (8th U) | 3 | 23.1 |
| 1211 | Oligo(U) (10th U) | 1 | 7.7 |
| 1207 | Oligo(U) (5th U) | 1 | 7.7 |
| Total | 77.0 | ||
| 1011 | IRE (minus-strand) | 2 | 15.4 |
| 1014 | IRE (minus-strand) | 1 | 7.7 |
a
The 3′ termini were determined by sequencing of RT-PCR-generated cDNA clones from copying reaction products of minus-strand RNA3 templates, as specified in Fig. 3 and described in Materials and Methods.
b
The numbers refer to nucleotide positions in the published sequence of BMV RNA3 (1).
c
INT, intergenic region.