FIG. 5.

Autointegration events recovered using wild-type or cPPT-defective (cppt-74) Ty1 elements containing the Zeo cassette. (A) The positions of the Ty1 LTRs (triangles), the cPPT, and autointegration events resulting in deletion circles (short solid lines) and inversion circles (short dotted lines with diamonds) are shown. Ty1 sequence coordinates are shown below. The resolution of the figure is about 10 bp (see file S2 in the supplemental material for nucleotide positions of the autointegration events). (B) Consensus sequence of the 5-bp target site duplication for Ty1 autointegration events constructed using WebLogo (16). The size of the nucleotide at each position reflects the frequency with which that nucleotide occurs in the target site. Target sites are oriented in the 5′ to 3′ direction from the end of the LTR adjacent to the Zeo cassette. Error bars are twice the small sample correction.