TABLE 2.
Strain genotype | Mean Ty1his3-AI retrotransposition rate (SD) (10−6)
|
Fold decrease | |
---|---|---|---|
WT | cppt-74 | ||
WT | 6.7 (1.0) | 2.7 (0.7) | 2.5 |
rad52Δ | 8.75 (1.0) | 5.0 (0.5) | 1.75 |
The centromere-based plasmid pBDG996 containing a wild-type Ty1his3-AI or the cppt-74 mutant were introduced into wild-type and rad52Δ Ty1-less strains, and the rate of His+ colony formation was determined as previously described (17). The rate of His+ formation was the average of two independent experiments.