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. 2006 Oct 11;80(24):12312–12323. doi: 10.1128/JVI.01766-06

FIG. 3.

FIG. 3.

Expression of UL28 protein from wild-type HSV-1 and UL28 mutant viruses. Vero cells were infected with the indicated virus at an MOI of 5 PFU per cell. At 18 h postinfection, cell lysates were prepared, separated by SDS-PAGE, and immunoblotted. The expression of the UL28 protein was detected using a polyclonal antisera raised against an Escherichia coli-expressed Cro-UL28 fusion protein (35). The left panel shows the insertion mutants. The right panel shows the stop mutants, and the locations of the UL28-specific polypeptides expressed from these mutants are indicated by arrowheads.