FIG. 6.

Transcription profiling of KSHV ORF58, ORF59, ORF60, ORF61, and ORF62 by Northern blotting. (A) Schematic diagram of predicted positions and transcription orientations of the ORFs from nt 94471 to 101194 in the KSHV genome. Dashes below the 6.7-kb transcript are individual ORF-specific probes used in this study. (B and C) Northern blot analysis. Approximate 1 μg of poly(A)⁺ mRNAs were isolated from uninduced (lane 1 in panel B and lanes 1, 3, 5, 7, and 9 in panel C) or butyrate-induced (lane 2 in panel B and lanes 2, 4, 6, 8, and 10 in panel C) JSC-1 cells, separated in a 1% formaldehyde-morpholinepropanesulfonic acid agarose gel, transferred onto a nylon membrane, fixed with UV light, and probed with ³²P-labeled antisense probe e prepared by in vitro transcription (B) (see Fig. 4A) or a ³²P-labeled oligonucleotide prepared by end labeling (C).