FIG. 3.

Characterization of PRV lacking the VP1/2 amino terminus. (A) Single-step propagation kinetics. The following viruses were harvested from the medium (Sups) (dashed lines and open symbols) and PK15 cells (solid lines and filled symbols) at the indicated times: PRV-GS847 (wild type [WT] VP1/2; circles), PRV-GS1651 (Δ6-225 VP1/2; squares), and PRV-GS1651R (revertant [Rev] VP1/2; triangles). (B) Rates of retrograde capsid transport in axons following infection of primary sensory neurons in culture. Error bars are standard errors of the means. (C) Frequency of de novo assembled capsids observed in axons of cultured sensory neurons follow replication (20 hpi). (D) Western blot analysis of the level of expression of the UL37 tegument protein in infected cells and released extracellular virions.