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Expression of VP1/2 fused to GFP in Vero cells. Cells transfected with either GFP-VP1/2(aa3023-3084), GFP-VP1/2(aa2-3084), or unfused GFP are shown. (A) Uninfected cells; (B) cells subsequently infected with PRV expressing red-fluorescent capsids. Images were captured from a basal focal plane of the cells, thereby revealing capsid assemblies near the bottom of the nucleus. DIC, differential interference contrast.
