TABLE 2.

Levels of acute viral replication and numbers of latent viral genomes in brain stems and other brain regions

Tissue examined	Viral titer in tissue at day p.i.a:			No. of latent viral genomes at day 30 p.i.b
	3	5	7
Brain stem	2.2 ± 0.3 (5)	4.1 ± 0.1 (5)	1.6 ± 0.1 (5)	5.9 ± 0.1 (10)
Cerebellum	0.5 ± 0.3 (5)	1.6 ± 0.3 (5)	0.7 ± 0.3 (5)	1.6 ± 0.4 (10)
Olfactory bulbs	0 (5)	1.3 ± 0.5 (5)	0 (5)	3.9 ± 0.3 (6)
Frontal cortex	0 (5)	0.5 ± 0.3 (5)	0.3 ± 0.3 (5)	4.2 ± 0.4 (6)
Hippocampus	0 (5)	0.7 ± 0.5 (5)	0 (5)	3.2 ± 1.1 (6)

^aBALB/c mice were infected with 2 × 10⁷ PFU of HSV-1 strain KOS via both eyes, and brain tissues were harvested at indicated times p.i. for titration of virus. Results are expressed as the log₁₀ mean PFU ± standard error of the mean/100 mg tissue for each group. Data were collected from at least two independent experiments, and the number of samples assayed for each group is indicated in parentheses.

^bAt day 30 p.i., brain tissues were harvested and processed to quantify viral genomes by quantitative real-time PCR. Results are expressed as the log₁₀ mean number of genomes ± standard error of the mean/μg DNA for each group. Data were collected from at least two independent experiments, and the number of samples assayed for each group is indicated in parentheses.