TABLE 1.

Inhibition of Ad attachment by CD46 antibodies^a

Cell type and name	% Inhibition of binding by:
	Ad3	Ad7p	Ad11p	Ad14	Ad16	Ad21	Ad35	Ad50
Tumor
HeLa	42.8	34.6	57.4	44.1	92.4	89.8	90.6	89.6
K562	33	33.8	72.4	38.7	95.4	92.2	93.4	92.8
Hep-2	39		64.8	40.9			84.5
Skov3	28.5		76				83.1
293 LP	0.4		41.7				89.4
Huh7	47.7		79.9				93.7
Stem
MES	11.2		57.1				90.7	90.2
hSF6	20.7		41.2		95.9		81.1	82.2
CD34+	10.3	11.9	56.7				62.9
Normal
MHF2	6.1		81.4	21.4			89.3

^aCells were preincubated with anti-CD46 antibody MEM-258 or control antibody. ³H-labeled Ads were then added at an MOI of 8,000 VP per cell, and the number of VP bound per cell was determined after 1 h of incubation at 4°C as described in Materials and Methods. Cell types are grouped as transformed or tumor cell lines, primary stem cells, and primary normal cells. Shown is the percentage of inhibition of binding after preincubation with control versus CD46 antibody. All samples were analyzed in triplicate. Standard deviation was less than 10%.