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. 2006 Oct 11;80(24):12041–12048. doi: 10.1128/JVI.01425-06

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FIG. 4. — Interaction in vitro of recombinant TAF9 (aa 1 to 140) and rv-cyclin carboxyl regions. A. Pulldown of recombinant, His-tagged TAF9 by GST fusion proteins rv-cyclin aa 219 to 297 (GST-219-297), by wild-type GST-rv-cyclin AD (GST-240-270) (wt) and mutated forms V260F and V260S, and by wild-type GST-VP16C (wt) and corresponding mutated form, F475A. Western blots were probed with antibody reactive to the amino terminus of TAF9 (αTAF9) and reprobed with anti-GST (αGST; bottom panel), which recognizes the input GST fusion proteins. B. Reciprocal pulldown in vitro of wild-type His-tagged rv-cyclin aa 219 to 297 (His-219-297) (wt) and aa 240 to 270 (His-240-270) (wt) and mutated forms of each protein, V260S and V260F, with GST-TAF9 fusion protein (aa 1 to 140). The results of a control pulldown of wild-type His-240-270 (wt) with GST protein alone are shown in the first lane of the right panel. Western blots were probed with antibody reactive to poly-His (αHis) and reprobed with anti-GST (αGST; bottom panel), which recognizes the input GST and GST-TAF9 fusion protein.