FIG. 5.

Binding of K8, RTA, and core replication proteins to KSHV ori-Lyt DNA. (A) Schematic of KSHV ori-Lyt core domain and the three DNA fragments that were used in the DNA affinity assay. The C/EBP cluster (K8 binding sites), AT palindrome (AT Pal), RRE, and TATA boxes are illustrated. (B) Nuclear extract (NE) from TPA-induced BCBL-1 cells was subjected to DNA affinity purification with each of the three DNA probes, and the D500 eluates were assayed by Western blotting with antibodies as indicated. An irrelevant DNA (ORF45 coding region) was included as a control, C.