FIG. 6.

Effect of deletion of RRE from the ori-Lyt fragment (11F) on the recruiting of replication proteins to ori-Lyt DNA. (A) Sequences of 11F and 11FΔRRE DNA fragments used in the DNA affinity assay. (B) Biotinylated DNA fragments were prepared by PCR, with pOri-A (wild-type) DNA and pOri-Δ15.7 (an RRE deletion derivative, which was described by Wang et al. [31]) as templates. TPA-induced BCBL-1 nuclear extract (NE) was incubated with the DNA fragments conjugated on magnetic beads, washed, and eluted with D500 elution buffer. Samples were assayed by Western blotting with antibodies as indicated. An irrelevant DNA was included as a control, C.