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. 2006 Oct 4;80(24):12141–12148. doi: 10.1128/JVI.01648-06

FIG. 3.

FIG. 3.

Microglia are not activated in cocultures with BDV-infected primary cortical neurons. (A) Primary cortical neurons were mock- or BDV-infected and immunostained for a neuronal marker, MAP2 (green), and BDV (red) on day 12 p.i. About 60% to 90% of neurons were infected on day 12 in vitro. (B) BDV- or mock-infected primary cortical neurons were cocultured with microglia on day 12 p.i. and immunostained for a microglia marker after 3 days of coculturing (lectin-IB4; red), neurons (MAP2; green) and for MHC-I (white). Cocultures of cortical neurons with microglia were not associated with activation of microglia by BDV-infected neurons. Note the absence of differences in the microglia morphology between the infected and control cultures. Scale bar, 40 μm.