FIG. 4.

RSV F protein membrane-proximal palmitoylation, TM domain, and CTD are not required for lipid raft association. (A) SynF sequences for TM domain and CTD mutants are listed. The final construct is a chimeric protein between the VSV G TM domain and CTD and the ectodomain of F protein. *, palmitoylation site. (B) Cells were transfected with the RSV F protein mutants, and colocalization of GM1 and the altered F proteins was analyzed by confocal microscopy. Labeling was done as described in the legend for Fig. 3, and cells were analyzed by confocal microscopy. The portions indicated by the boxes are magnified approximately ×5 in the insets.