FIG. 5.

DRM association of F protein with mutations to the palmitoylation site, TM domain, and CTD. Detergent solubility of RSV F protein was examined in cells transfected with the various F mutation constructs or with VSV G. Postnuclear supernatants were treated with 0.25% Triton X-100 at 4°C and separated on a density gradient. Subsequent blots of the fractions were probed with either an anti-RSV F antibody or an antibody specific for the CTD of VSV G (anti-VSV G), as specified to the right of the respective blots. The SynF mutant is labeled to the left of the blot. Also shown are representative blots probed with anti-Cav-1 and anti-TfR.