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. 2006 Oct 4;80(24):12332–12342. doi: 10.1128/JVI.01325-06

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Copyright © 2006, American Society for Microbiology

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FIG. 4. — LGP2 coprecipitates with IPS-1 and RIG-I. (A) HEK 293T cells were transfected with the expression vector for Myc-tagged RIG-I and the expression vector for FLAG-tagged IPS-1 along with the expression vector for His-tagged LGP2 or His-tagged Yap. Cell lysates were subjected to FLAG immunoprecipitation using M2-agarose beads and immunoblotting for Myc or His tags. (B) HEK 293T cells were transfected with the expression vector for Myc-tagged RIG-I and the expression vector for FLAG-tagged IPS-1 along with increasing amounts of the expression vector for His-tagged LGP2. Lysates were subjected to FLAG immunoprecipitation as for panel A. (C) HEK 293T cells were transfected with the expression vector for His-tagged RIG-IC or LGP2 along with the expression vector for FLAG-tagged IPS-1 or empty vector. Lysates were subjected to FLAG immunoprecipitation followed by immunoblotting, using His-tag antibody. (D) Lysates from Sendai virus-infected HEK 293T cells were subjected to immunoprecipitation with LGP2 antibody or control rabbit IgG. The precipitated proteins were analyzed by anti-Cardif antibody, and the same blot was reprobed with LGP2 antibody.