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. 2006 Oct 4;80(24):12332–12342. doi: 10.1128/JVI.01325-06

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Copyright © 2006, American Society for Microbiology

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FIG. 7. — LGP2 competes with IKKi for IPS-1binding. (A) HEK 293T cells were transfected with expression vectors for FLAG-tagged IKKi and GST-IPS-1 fusions, as indicated. Cell lysates were subjected to glutathione bead affinity purification. FLAG-tagged IKKi copurification with GST-IPS-1 was analyzed by immunoblotting, using FLAG antibody. (B) HEK 293T cells were transfected with expression vectors for FLAG-tagged IKKi and HA-tagged IPS-1 along with increasing amounts of the expression vector for six-His-tagged LGP2. Cell lysates were subjected to HA purification. FLAG-tagged IKKi purified with HA-IPS-1 was analyzed by immunoblotting, using FLAG antibody.