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Rearranged μ1 is a helical protein. (A) CD spectrum of rearranged μ1 at room temperature. (B) CD melting curve of rearranged μ1 at 222 nm. (C) Limited proteolysis of rearranged μ1. μ1* was treated with increasing amounts of trypsin; the mass ratio of trypsin to μ1* is indicated for each lane (first lane from left, untreated μ1*; last lane from left, trypsin alone). Rearranged μ1 and a relatively stable fragment of μ1* are indicated by arrows.
