FIG. 1.

Construction of the Gag dAL11 DNA vaccine. (A) The Gag dAL11 DNA vaccine was produced by mutating the position 5 anchor residue of the AL11 epitope from asparagine (N) to alanine (A). (B) Expression of Gag WT and Gag dAL11 DNA vaccines was assessed in transiently transfected 293 cells by enzyme-linked immunosorbent assay. (C) AL11-specific CD8⁺ T-lymphocyte responses were evaluated by D^b/AL11 tetramer binding assays of C57BL/6 mice immunized with Gag WT and Gag dAL11 DNA vaccines. Error bars indicate standard errors.