FIG. 2.

Cellular immune responses elicited by Gag WT and Gag dAL11 DNA vaccines. Groups of C57BL/6 mice (n = 12/group) were immunized i.m. at week 0 and week 4 with 50 μg Gag WT or Gag dAL11 DNA vaccine. AL11- and KV9-specific CD8⁺ T-lymphocyte responses were assessed by IFN-γ ICS assays. (A) Representative mice at week 2. (B) Mean responses in both groups at multiple time points following immunization. (C) IFN-γ ELISPOT assays in response to a pool of overlapping Gag peptides, the CD4⁺ T-lymphocyte epitope DD13, and the CD8⁺ T-lymphocyte epitopes AL11 and KV9 at week 4 and week 8. SFC, spot-forming cells. Error bars indicate standard errors.