FIG. 4.

Anatomic separation of Gag WT and Gag dAL11 DNA vaccines. Groups of C57BL/6 mice (n = 8/group) were immunized once i.m. with the following DNA vaccine regimens: 50 μg Gag WT (half the dose in each leg), 50 μg Gag dAL11 (half the dose in each leg), 25 μg Gag WT and 25 μg Gag dAL11 mixed together in the same syringe (half the dose in each leg), or 25 μg Gag WT (left leg) and 25 μg Gag dAL11 (right leg). AL11- and KV9-specific CD8⁺ T-lymphocyte responses were assessed by IFN-γ ICS assays at week 2 following immunization. Error bars indicate standard errors.