FIG. 6.

Characterization of MAb M6 binding to linear epitopes on the GBV-C E2 protein. (A) The E2 coding sequences were expressed in E. coli, and protein expression was detected by Western blot assay with anti-Xpress or M6 antibodies as shown. NC, negative control (E. coli without GBV-C E2 protein); M, marker. (B) ELISA detection of M6 binding to overlapping 13-mer peptides with or without the biotinylation adaptor (Bi) and a 17-mer peptide containing the entire epitope. Removal of Bi resulted in decreased binding to L13C and loss of binding of G13L.