FIG. 1.
Series of parental and mutant cDNAs. The position of the TMEV coding area is shown at the top. pDAFL3 and pGDVIIFL2 are parental cDNAs derived from DA and GDVII, respectively. DA and GDVII genomes are shown as open and solid bars, respectively. pDAL*-1 has a mutation changing the initiation codon AUG of L* to ACG; therefore, it fails to synthesize L*. Mutant constructs DALPro and DALPro/L*-1 were generated by introducing a mutation at nt 1234, leading to a predicted change in the amino acid residue at position 57 (L57) from the serine of DA to proline. Another mutant construct, GDVIILSer, was generated by introducing a mutation into nt 1236, leading to a predicted change in L57 from the proline of GDVII to serine. UTR, untranslated region.