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Kinetics of cognate DNA cleavage by wild-type and variants of PI-MtuI. Reactions containing wild-type (A) or variants (B and C) of PI-MtuI were performed as described in Materials and Methods. (D) Quantification of the extent of cognate DNA cleavage by wild-type and variants of PI-MtuI shown in (A–C). The graph shows the mean of two independent experiments. Form I, negatively supercoiled DNA; Form II, nicked circular DNA; Form III, linear double-stranded DNA.
