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(A) Comparison of fluorescence spectral characteristics of 2 µM 7AM-ACTD in the absence and in the presence of 40 µM d(CTAGGCCTAG) with excitation wavelength at 560 nm. (B) Comparison of fluorescence kinetic traces at 20°C for the binding of 7AM-ACTD to d(CTAGGCCTAG) (monitored at 600 and 660 nm) and to d(ATAGGCCTAT) (monitored at 660 nm) with 560-nm excitation.
