Table 3. Relative quantitation of known concentrations of β-actin plasmid and cDNA.
Sample | Dilution | R0 | Calculated dilution |
---|---|---|---|
β-Actin plasmid | 1 000 000 copies | 4.063 × 10–7 | 1.000 |
100 000 copies | 4.386 × 10–8 | 0.108 | |
10 000 copies | 3.299 × 10–9 | 0.008 | |
1000 copies | 2.620 × 10–10 | 0.001 | |
β-Actin cDNA | 1 | 8.805 × 10–8 | 1.000 |
0.1 | 9.200 × 10–9 | 0.104 | |
0.01 | 8.618 × 10–10 | 0.010 |
Dilutions are calculated from the R0 of each dilution as a fraction of the R0 of the undiluted sample.