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Partial digestion of pUC19 plasmid DNA with TspDTI restriction endonuclease. (A) TspDTI cleavage of pUC19 DNA, 1.5% agarose/TAE. Lane 1, 1 kb ladder; lane 2, 100 bp ladder; lane 3, untreated pUC19 DNA; lane 4, TspDTI-cut pUC19 DNA. (B) TspDTI cleavage of pUC19 DNA, 6% polyacrylamide/TBE. Lane 1, 100 bp ladder; lane 2, untreated pUC19 DNA; lane 3, TspDTI-cut pUC19 DNA. The smallest partial digestion band of 376 bp is indicated in bold italics with horizontal arrow.
