Table 2. Comparison between TspDTI/TspGWI endonuclease ‘twins’ and class-IIS restriction endonucleases with related recognition and cleavage sites.
| Restriction endonucleasea | Bacterial hosta | Recognition sitea | Cleavage positionsa | Reaction temperaturea | Polypeptide sizeb | Native molecular sizec | Specific DNA methylation | Reference |
|---|---|---|---|---|---|---|---|---|
| TspDTI | Thermus sp. | ATGAA | N11/9 | 70°C | 114.5 kDa | 110–130 kDa | +++ | This work |
| TspGWI | Thermus sp. | ACGGA | N11/9 | 70°C | 122.0 kDa | 110–130 kDa | +/– | (32), This work |
| TaqII | Thermus aquaticus | GACCGA | N11/9 | 70°C | 118.5 kDa | 110–130 kDa | ++++ | (33), (36), This work |
| CACCCA | N11/9 | (125.6 kDad) | ||||||
| Tth111II | Thermus thermophilus | CAARCA | N11/9 | 70°C | 116.5 kDa | 110–130 kDa | ND | (34), This work |
| EciI | Escherichia coli | GGCGGA | N11/9 | 37°C | ND | ND | ND | REBASE |
| BcefI | Bacillus cereus | ACGGC | N12/13 | 30°C | ND | ND | ND | (35) |
| BceAI | Bacillus cereus | ACGGC | N12/14 | 30°C | ND | ND | ND | REBASE |
aThermus sp.-derived and TspDTI/TspGWI-related endonucleases, bases in a recognition sequence, cleavage positions and reaction temperatures are marked in bold.
bAs estimated by SDS/PAGE of homogeneous proteins.
cAs estimated by molecular sieving under native buffer conditions.
dAs calculated from sequencing/genetic analysis data obtained for the taqIIR gene.
ND, not determined.