Figure 1.

Visualization of SCD degradation by expression of the SCD-GFP construct. (A) CHO cells were transfected with vector expressing the GFP alone or SCD 1–358-GFP construct. After 48 h, the transfected cells were treated with cycloheximide 10 μg/ml for 0, 2, and 4 h and their fluorescence determined. (B) Flow cytometric analysis of the fluorescence stabilities of GFP and SCD 1–358-GFP constructs. CHO cells were transfected with the constructs and after 48 h, cells were treated with cycloheximide for 0, 2, and 4 h. The treated cells were collected, and 10,000 cells were subjected to FACS analysis. The percentage of cells deemed fluorescent is indicated in the figure.