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. 2000 Aug 1;97(16):8883–8888. doi: 10.1073/pnas.97.16.8883

Figure 2.

Figure 2

Deletion of the N terminus of SCD inhibits the degradation of SCD-GFP constructs. (A) Fluorescence of SCD 27–358-GFP and SCD 45–358-GFP after cycloheximide treatment. The constructs localized to the ER remained fluorescent without relocalization to other cellular compartments. (B) A flow cytometric analysis of the fluorescence stabilities of SCD-GFP constructs with deleted N termini. After 48 h, the transfected cells were treated with cycloheximide for 0, 2, and 4 h. The treated cells were collected, and 10,000 cells were subjected to FACS analysis. The percentage of cells deemed fluorescent is indicated.