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. 2000 Aug 1;97(16):8883–8888. doi: 10.1073/pnas.97.16.8883

Figure 4.

Figure 4

Immunoblot analysis of the degradation of SCD-GFP constructs and appropriate translation initiation of SCD 27–358-GFP. GFP and derivatives of SCD-GFP were expressed in CHO cells and treated with cycloheximide. The cell lysates were subjected to SDS/PAGE and transferred onto Immobilon-P membrane. (A) Immunoblot of SCD-GFP fusion proteins with a polyclonal antibody against SCD. (B) Immunoblot with monoclonal antibody against GFP. (C) Immunoblot analysis of cells expressing GFP, SCD 1–33-GFP, and SCD 1–27-GFP with monoclonal antibody against GFP. (D) Resolution of SCD 27–358-GFP and SCD 34–358-GFP after 15% SDS/PAGE and immunoblot with monoclonal antibody against GFP.