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. 2000 Aug 1;97(16):8883–8888. doi: 10.1073/pnas.97.16.8883

Figure 5.

Figure 5

Mutation of lysine to alanine at position 33 [SCD 1–33(K33/A)-GFP] leads to a more stable chimera. (A) Fluorescence of SCD 1–33(K33/A)-GFP before and after cycloheximide treatment. (B) A flow cytometric analysis of the fluorescence stability of the mutated construct. The percentage of cells deemed fluorescent is indicated. The table shows fluorescent cells for each time point as a percentage of initial fluorescence. (C) Immunoblot analysis of cells expressing SCD 1–33(K33/A)-GFP with monoclonal antibody against GFP after treatment with cycloheximide. (D) Immunoblot analysis of the mutant in cellular subfractions. The whole cell extract (W), pellet fraction (P), and soluble fraction (S) are shown.