Figure 10.

Degradation of unfolded GFP and rhodanese by ClpAP. Acid-denatured [³H]GFP (blue bars), native [³H]GFP (blue hatched bars), acid-denatured [³H]rhodanese (yellow bars), and native [³H]rhodanese (hatched yellow bars) were incubated as described in the text with ClpAP and 1 mM ATP[γS] for 5 min to bind the unfolded protein. Then 10 mM ATP was added and after 20 min, degradation was measured. Where indicated, ATP, ClpP, ClpA, or ATP[γS] was omitted. Results are means (±SEM) of three independent experiments.