Figure 7.
Translocation of unfolded GFP-SsrA to ClpP. (A) ClpXPin or ClpAPin was assembled by incubating ClpX (1 μM) or ClpA (1 μM) for 30 sec with 1 μM DIP-ClpP and 5 mM ATP (plus regenerating system). Fluorescence was monitored after addition of 1 μM GFP-SsrA to the ClpXPin complexes (▵) or the ClpAPin complexes (▴). (B) GFP-SsrA (5 μM) was unfolded with ClpXPin made with 1 μM ClpX and 1.2 μM inactive ClpP-CMK (○). After the fluorescence decrease reached a plateau, 10 μM GroEL-trap was added (●).