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Myotubularin is localized to the cytoplasm in HEK293 cells. The subcellular distribution of transiently expressed EGFP (a) and EGFP-myotubularin fusion protein (EGFP-MTM1) (b) was determined by using immunofluorescence microscopy. Nuclei were stained with DAPI (c and d). An identical pattern of cytoplasmic staining for EGFP and EGFP-MTM1 or with FLAG-tagged myotubularin was observed in both HeLa cells and murine C2C12 myoblasts (not shown).
