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Deacylation of G1:C72/U70-Ala-tRNAPro by wild-type (WT) and C443G ProRS. (A) Deacylation by 0.1 μM C443G-ProRS (■) and wild-type ProRS (●). (B) Deacylation by 0.5 μM (■), 1 μM (▴), and 2 μM (▾) C443G-ProRS. In both graphs, an assay performed in the absence of ProRS (⧫) is shown.
