Figure 2.

Phg2 mutant cells initiate multicellular development in the presence of abnormally high concentrations of nutrients. (A and B) Wild-type or mutant cells were placed for 24 h in a medium containing a defined amount of nutrients, obtained by diluting HL5 medium with phosphate buffer. After 24 h, multicellular development was monitored by assessing the presence of tight cellular aggregates. Bar, 200 μm. +, presence of tight cellular aggregates; −, absence of aggregates. Phg2 mutant cells initiated multicellular development at concentrations of nutrients that inhibited development of wild-type or myoVII cells. (C) Cells treated as in A were harvested and the expression of csA was determined by Western blot analysis.