Figure 7.

Initiation of multicellular development is altered in adrm1 mutant cells. (A and B). Adrm1 mutant cells were generated in an AX2 Dictyostelium strain. Wild-type or mutant cells were incubated in medium containing defined amounts of nutrients, and multicellular development was determined by assessing the formation of multicellular aggregates (A) and the expression of csA (B), as described in Figure 2. (C) The expression of discoidin I was assessed in cells grown at low cellular densities (below 10⁶ cells/ml). In these conditions, discoidin was overexpressed in adrm1 mutant cells compared with wild-type cells.