Table 1.
Yeast strains
| Strain | Genotype | Source |
|---|---|---|
| W303-1A | MATa ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 | R. Rothstein |
| W303-1B | MATαade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 | R. Rothstein |
| NY179 | MATa leu2-3,112 ura3-52 | P. Novick |
| BY4741 | MATa his3 leu2 ura3 | Euroscarf |
| WKL-2A | MATa ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 sro7::LEU2 | Larsson et al. (1998) |
| WKL-2B | MATa ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 sro7::LEU2 | Larsson et al. (1998) |
| Y05451 | BY4741 sro7:: kanMX4 | Euroscarf |
| Y00852 | BY4741 rsn1:: kanMX4 | Euroscarf |
| YN10 | MATa ade2oc can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 ena1::HIS3 | Wieland et al. (1995) |
| WKL-210 | YN10 sro7::LEU2 | This study |
| YIW-2 | W303-1B ENA1-3xHA | This study |
| YIW-3 | W303-1B pep4::kanMX4 ENA1-3xHA | This study |
| YIW-4 | WKL-2A ENA1::3xHA | This study |
| YIW-5 | WKL-2A pep4::kanMX4 ENA1-3xHA | This study |
| YIW-18 | BY4741 end4::kanMX4 sro7::LEU2 | This study |
| YIW-19 | BY4741 vps27:: kanMX4 sro7::LEU2 | This study |
| YIW-20 | YIW-18 ENA1-3xHA | This study |
| YIW-21 | YIW19 ENA1-3xHA | This study |
| YIW-22 | NY786 sro7::LEU2 | This study |
| YIW-23 | YIW-22 ENA1-3xHA | This study |
| YCS-1 | W303-1B RSN1-CFP | This study |
| RSY255 | MATα ura3-52 leu2-3,112 | R. Sheckman |
| NY772 | MATa leu2-3,112 ura3-52 sec3-2 | P. Novick |
| NY774 | MATα leu2-3,112 ura3-52 sec4-8 | P. Novick |
| NY776 | MATα leu2-3,112 ura3-52 sec5-24 | P. Novick |
| NY778 | MATα leu2-3,112 ura3-52 sec6-4 | P. Novick |
| NY780 | MATα leu2-3,112 ura3-52 sec8-9 | P. Novick |
| NY782 | MATa leu2-3,112 ura3-52 sec9-4 | P. Novick |
| NY784 | MATa leu2-3,112 ura3-52 sec10-2 | P. Novick |
| NY786 | MATa leu2-3,112 ura3-52 sec15-1 | P. Novick |
| H1394 | NY179 sro7::URA3 | This study |
| H1295 | NY772 sro7::URA3 | This study |
| H1396 | NY774 sro7::URA3 | This study |
| H1296 | NY776 sro7::URA3 | This study |
| H1297 | NY778 sro7::URA3 | This study |
| H1298 | NY780 sro7::URA3 | This study |
| H1397 | NY782 sro7::URA3 | This study |
| H1299 | NY784 sro7::URA3 | This study |
| H1300 | NY786 sro7::URA3 | This study |
| 27061b | MATa ura3 trp1 | Galan et al. (1996) |
| 27064b | MATa ura3 trp1npi1 | Galan et al. (1996) |
| npi1sro7 | MATa ura3 trp1npi1 sro7::kanMX4 | This study |