Figure 3.

Binding duration of immobile U1 snRNPs. (A–D) The curves quantify the dissociation of U1 snRNPs from binding sites observed at 200, 100, 10, and 5 Hz as indicated in the graphs. The axis labels given at the left hand axis refer to the nucleoplasmic binding (○), and the labels at the right hand axis refer to the binding in speckles (•). The lines show the results of fits to the data using a sum of two exponentials. Dotted lines, the fits to the nucleoplasm data; solid lines, fits to the speckle data. (E) The averaged decay times as determined by the fits in A–D were determined and plotted as a function of the image cycle time. Obviously the binding times were related to the time scale, at which the binding was analyzed.