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. 2006 Dec;17(12):5075–5093. doi: 10.1091/mbc.E05-11-1009

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© 2006 by The American Society for Cell Biology

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Figure 2. — mod21p is a component of the fission yeast γ-TuC. (A and B) Anti-HA coimmunoprecipitation experiments from cell extracts expressing (A) Myc-tagged mod21p and (B) Myc-tagged gfh1p as well as HA-tagged alp4p or HA-tagged alp6p, as indicated. (C) Localization of GFP-tagged alp4p, gfh1p, mod21p, and alp16p (green in merge) in live cells coexpressing the SPB marker sad1-dsRed (red in merge), with DAPI counterstaining (blue in merge). In each set, interphase cells are shown above and mitotic cells below. Note interphase satellites of alp4-GFP (yellow arrow) and relatively weak eMTOC localization of gfh1p, mod21p, and alp16p relative to alp4p (white arrows). All nonenhanced images were collected and processed under identical conditions, allowing direct comparison of intensities. Enhanced images were individually altered to highlight faint eMTOC localization.