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Microtubule nucleation and SPB behavior in wild-type and mutant cells. Stills from movies imaging GFP-atb2p (expressed together with endogenous untagged atb2p) and sad1-dsRed, at 20-s intervals, in cells of the indicated genotypes (see Materials and Methods). Yellow arrows indicate representative (but not all) examples of nucleation from the SPB, and white arrows indicate nucleation from non-SPB
