Figure 7.

Microtubule bundle dynamics and SPB oscillations in wild-type and mutant cells. (A) Microtubule bundle appearance and lifetimes. Each graph shows the times of appearance and the lifetimes of microtubule bundles in a representative single cell of the indicated genotype, from a 30-min time-lapse sequence imaging both GFP-atb2 and sad1-dsRed (see Materials and Methods). Two cells are shown for each genotype, with mod22+ strains on the left-hand side and mod22-1 strains on the right-hand side. Microtubule bundles associated with the SPB are shown in red. (B) Schematic of how time-projection images were created to display SPB oscillations. (C) Average time-projection images of wild-type and mutant cells, from 30-min time-lapse sequences imaging both GFP-atb2 and sad1-dsRed. In wild-type and mod22-1 cells, only small movements are observed.