Table 1.
Fission yeast strains used in this study
| Strain | Genotype | Source |
|---|---|---|
| KS515 | ade6-M216 ura4-D18 leu1-32 h+ | Laboratory stock |
| KS516 | ade6-M210 ura4-D18 leu1-32 h− | Laboratory stock |
| KS959 | h− gfh1Δ::kanMX6 ade6-M216 leu1-32 ura4-D18 | This study |
| KS963 | h− gfh1Δ::kanMX6 mod22-1 ade6-M216 leu1-32 ura4-D18 | This study |
| KS1107 | h− mod21Δ::kanMX6 ade6-M210 leu1-32 ura4-D18 isolate 4 | This study |
| KS1121 | h− mod21Δ::kanMX mod22-1 ade6-216 leu1-32 ura4-D18 | This study |
| KS1138 | h− alp4-3HA::kanMX ade6-216 his- leu1-32 ura4-D18 | T. Toda (Vardy et al., 2000) |
| KS1139 | h− alp6-3HA::kanMX ade6-210 his- leu1-32 ura4-D18 | T. Toda (Vardy et al., 2000) |
| KS1140 | h− alp4-GFP::kanMX ade6-210 his- leu1-32 ura4-D18 | T. Toda (Vardy et al., 2000) |
| KS1225 | h+ ars1::nmt81::GFP-atb2::LEU2 ade6-210 leu1-32 ura4-D18 | This study |
| KS1236 | h− kanMX::nmt81::GFP-atb2 ade6-216 leu1-32 ura4-D18 | Laboratory stock (Sawin et al., 2004) |
| KS1362 | h− gfh1Δ::kanMX mod21Δ::kanMX mod22-1 ade6-M216 leu1-32 ura4-D18 | This study |
| KS1365 | h− gfh1Δ::kanMX mod21Δ::kanMX ade6-M216 leu1-32 ura4-D18 | This study |
| KS1433 | h− gfh1-GFP::kanMX ade6-M210 leu1-32 ura4-D18 | This study |
| KS1466 | h− mod21-13Myc::kanMX ade6-M210 leu1-32 ura4-D18 | This study |
| KS1470 | h− mod22-1 ade6-210 leu1-32 ura4-D18 | This study |
| KS1492 | h− gfh1-3HA::kanMX ade-M210 leu1-32 ura4-D18 | This study |
| KS1525 | h+ alp4-13Myc::kanMX ade6-M216 leu1-32 ura4-D18 | This study |
| KA1527 | h+ alp6-13Myc::kanMX ade6-M216 leu1-32 ura4-D18 | This study |
| KS1563 | h− mod21-GFP::kanMX ade6-210 leu1-32 ura4-D18 | This study |
| KS1583 | h− gfh1-13Myc::kanMX ade6-M210 leu1-32 ura4-D18 | This study |
| KS1595 | h− gfh1-13Myc::kanMX alp6-3HA::kanMX ade6-210 leu1-32 ura4-D18 | This study |
| KS1597 | h− mod21-13Myc::kanMX alp6-3HA::kanMX ade6-210 leu1-32 ura4-D18 | This study |
| KS1599 | h+ gfh1-13Myc::kanMX alp4-3HA::kanMX ade6-216 leu1-32 ura4-D18 | This study |
| KS1603 | h+ mod21-13Myc::kanMX alp4-3HA::kanMX ade6-210 leu1-32 ura4-D18 | This study |
| KS1665 | h− alp16Δ::natMX ade6-M210 leu1-32 ura4-D18 | This study |
| KS1673 | h− alp16Δ::natMX mod21Δ::kanMX ade6-210 leu1-32 ura4-D18 | This study |
| KS1677 | h− alp16Δ::natMX gfh1Δ::kanMX ade6-M210 leu1-32 ura4-D18 | This study |
| KS1696 | h− mod21Δ::kanMX kanMX:nmt81:GFP-atb2 ade6-210 leu1-32 ura4-D18 | This study |
| KS1701 | h− mod21Δ::kanMX ars1:nmt81:GFP-atb2:LEU2 ade6-210 leu1-32 ura4-D18 | This study |
| KS1704 | h− gfh1Δ::kanMX kanMX:nmt81:GFP-atb2 ade6-216 leu1-32 ura4-D18 | This study |
| KS1708 | h− gfh1Δ::kanMX ars1:nmt81:GFP-atb2:LEU2 ade6-210 leu1-32 ura4-D18 | This study |
| KS1713 | h− alp16Δ::natMX gfh1Δ::kanMX mod21Δ::kanMX ade6-M210 leu1-32 ura4-D18 | This study |
| KS1720 | h− gfh1Δ::kanMX mod22-1 kanMX:nmt81:GFP-atb2 ade6-216 leu1-32 ura4-D18 | This study |
| KS1724 | h− mod21Δ::kanMX mod22-1 kanMX:nmt81:GFP-atb2 ade6-210 leu1-32 ura4-D18 | This study |
| KS1727 | h− gfh1Δ::kanMX mod22-1 ars1:nmt81:GFP-atb2:LEU2 ade6-210 leu1-32 ura4-D18 | This study |
| KS1733 | h− mod21Δ::kanMX mod22-1 ars1:nmt81:GFP-atb2:LEU2 ade6-210 leu1-32 ura4-D18 | This study |
| KS1762 | h− alp16Δ::natMX gfh1Δ::kanMX mod22-1 ade6-M210 leu1-32 ura4-D18 | This study |
| KS1764 | h− alp16Δ::natMX mod21Δ::kanMX mod22-1 ade6-M210 leu1-32 ura4-D18 | This study |
| KS1768 | h+ alp16Δ::natMX6 gfh1Δ::kanMX6 mod21Δ::kanMX6 mod22-1 ade6-210 leu1-32 ura4-D18 | This study |
| KS1804 | h+ alp16Δ::natMX mod22-1 ade6-M210 leu1-32 ura4-D18 | This study |
| KS1812 | h− alp16Δ::natMX6 gfh1Δ::kanMX6 mod21Δ::kanMX6 mod22-1 kanMX6:nmt81:GFP-atb2 ade6-210 leu1-32 ura4-D18 | This study |
| KS1817 | h− alp16Δ::natMX6 gfh1Δ::kanMX6 mod21Δ::kanMX6 mod22-1 ars1:nmt81:GFP-atb2:LEU2 ade6-210 leu1-32 ura4-D18 | This study |
| KS1900 | h+ gfh1-13Myc:kanMX6 ade6-216 leu1-32 ura4-D18 | This study |
| KS1908 | h+ mod21-13Myc:kanMX6 ade6-216 leu1-32 ura4-D18 | This study |
| KS1960 | h+ gfh1-3HA:kanMX6 ade6-210 leu1-32 ura4-D18 | This study |
| h− gfh1-13Myc:kanMX6 ade6-216 leu1-32 ura4-D18 | ||
| KS1961 | h− gfh1-13Myc::kanMX ade6-M210 leu1-32 ura4-D18 | This study |
| h+ ade6-M216 ura4-D18 leu1-32 | ||
| KS2003 | h+ mod21-13Myc::kanMX alp6-3HA::kanMX mod22-1 ade6-210 leu1-32 ura4-D18 | This study |
| KS2045 | h− mod21-13Myc::kanMX6 alp4-3HA::kanMX6 mod22-1 leu1-32 ura4-D18 | This study |
| KS2061 | h+ gfh1-3HA:kanMX6 mod21-13Myc::kanMX6 ade6-210 leu1-32 ura4-D18 | This study |
| KS2063 | h− gfh1Δ::hphMX6 mod21-13Myc::kanMX6 alp6-3HA::kanMX6 ade6-210 leu1-32 ura4-D18 | This study |
| KS2088 | h− mod21-13Myc::kanMX alp4-3HA::kanMX gfh1Δ::hphMX6 alp16Δ::natMX6 ade6-216 leu1-32 ura4-D18 | This study |
| KS2091 | h− mod21-13Myc::kanMX alp4-3HA::kanMX gfh1Δ::hphMX6 ade6-216 leu1-32 ura4-D18 | This study |
| KS2094 | h− mod21-13Myc::kanMX alp4-3HA::kanMX alp16Δ::natMX6 ade6-216 leu1-32 ura4-D18 | This study |
| KS2097 | h− mod21-13Myc::kanMX alp6-3HA::kanMX gfh1Δ::hphMX6 alp16Δ::natMX6 ade6-216 leu1-32 ura4-D18 | This study |
| KS2099 | h− mod21-13Myc::kanMX alp6-3HA::kanMX alp16Δ::natMX6 ade6-210 leu1-32 ura4-D18 | This study |
| KS2121 | h− mod21-13Myc::kanMX6 alp4-3HA::kanMX6 gfh1Δ::hphMX6 alp16Δ::natMX6 mod22-1 leu1-32 ura4-D18 | This study |
| KS2122 | h− gfh1-13Myc::kanMX6 alp4-3HA::kanMX6 mod21Δ::hphMX6 alp16Δ::natMX6 ade6-216 leu1-32 ura4-D18 | This study |
| KS2125 | h+ gfh1-13Myc::kanMX6 alp4-3HA::kanMX6 mod21Δ::hphMX6 ade6-216 leu1-32 ura4-D18 | This study |
| KS2127 | h+ gfh1-13Myc::kanMX6 alp4-3HA::kanMX6 alp16Δ::natMX6 ade6-216 leu1-32 ura4-D18 | This study |
| KS2133 | h− mod21-13Myc::kanMX6 alp4-3HA::kanMX6 gfh1Δ::hphMX6 mod22-1 ade6-216 leu1-32 ura4-D18 | This study |
| KS2134 | h− mod21-13Myc::kanMX6 alp4-3HA::kanMX6 alp16Δ::natMX6 mod22-1 ade6-216 leu1-32 ura4-D18 | This study |
| KS2137 | h− mod21-13Myc::kanMX6 alp6-3HA::kanMX6 gfh1Δ::hphMX6 mod22-1 ade6-210 leu1-32 ura4-D18 | This study |
| KS2141 | h− gfh1-13Myc::kanMX alp4-3HA::kanMX mod22-1 ade6-216 leu1-32 ura4-D18 | This study |
| KS2143 | h+ gfh1-13Myc::kanMX alp6-3HA::kanMX mod22-1 ade6-210 leu1-32 ura4-D18 | This study |
| KS2144 | h+ gfh1-13Myc::kanMX alp6-3HA::kanMX mod21Δ::hphMX6 ade6-210 leu1-32 ura4-D18 | This study |
| KS2146 | h− gfh1-13Myc::kanMX alp6-3HA::kanMX alp16Δ::natMX6 ade6-210 leu1-32 ura4-D18 | This study |
| KS2149 | h+ gfh1-13Myc::kanMX alp6-3HA::kanMX mod21Δ::hphMX6 alp16Δ::natMX6 ade6-210 leu1-32 ura4-D18 | This study |
| KS2163 | h− mod21-13Myc::kanMX alp6-3HA::kanMX gfh1Δ::hphMX6 alp16Δ::natMX6 mod22-1 ade6-216 leu1-32 ura4-D18 | This study |
| KS2164 | h+ mod21-13Myc::kanMX alp6-3HA::kanMX alp16Δ::natMX6 mod22-1 ade6-210 leu1-32 ura4-D18 | This study |
| KS2171 | h− gfh1-13Myc::kanMX alp4-3HA::kanMX alp16Δ::natMX6 mod22-1 ade6-216 leu1-32 ura4-D18 | This study |
| KS2172 | h− gfh1-13Myc::kanMX alp4-3HA::kanMX mod21Δ::hphMX6 mod22-1 ade6-216 leu1-32 ura4-D18 | This study |
| KS2175 | h− gfh1-13Myc::kanMX alp4-3HA::kanMX mod21Δ::hphMX6 alp16Δ::natMX6 mod22-1 ade6-216 leu1-32 ura4-D18 | This study |
| KS2177 | h+ gfh1-13Myc::kanMX alp6-3HA::kanMX alp16Δ::natMX6 mod22-1 ade6-210 leu1-32 ura4-D18 | This study |
| KS2178 | h+ gfh1-13Myc::kanMX alp6-3HA::kanMX mod21Δ::hphMX6 mod22-1 ade6-210 leu1-32 ura4-D18 | This study |
| KS2179 | h− gfh1-13Myc::kanMX alp6-3HA::kanMX mod21Δ::hphMX6 alp16Δ::natMX6 mod22-1 ade6-210 leu1-32 ura4-D18 | This study |
| KS2260 | h− sad1-dsRed:LEU2 leu1 (sad1+) | O. Niwa/T. Toda (Chikashige et al., 2004) |
| KS2362 | h− gfh1Δ::kanMX6 mod22-1 alp4-GFP::natMX ade6-M216 leu1-32 ura4-D18 | This study |
| KS2363 | h+ gfh1Δ::kanMX6 alp4-GFP::natMX ade6-M216 leu1-32 ura4-D18 | This study |
| KS2402 | h− mod21Δ::kanMX6 mod22-1 alp4-GFP::natMX ade6-M216 leu1-32 ura4-D18 | This study |
| KS2405 | h− mod21Δ::kanMX6 alp4-GFP::natMX ade6-M216 leu1-32 ura4-D18 | This study |
| KS2423 | h− alp16Δ::natMX gfh1Δ::kanMX mod21Δ::kanMX alp4-GFP::natMX ade6-210 leu1-32 ura4-D18 | This study |
| KS2508 | h− mod21-13Myc::kanMX6 alp16Δ::natMX ade6-M210 leu1-32 ura4-D18 | This study |
| KS2509 | h− gfh1-3HA::kanMX6 mod21-13Myc::kanMX6 alp16Δ::natMX ade6-M210 leu1-32 ura4-D18 | This study |
| KS2510 | h+ gfh1-3HA::kanMX6 mod21-13Myc::kanMX6 alp16Δ::natMX ade6-M210 leu1-32 ura4-D18 | This study |
| KS2519 | h− alp16-3HA::kanMX6 ade6-M210 leu1-32 ura4-D18 | This study |
| KS2520 | h− alp16-13Myc::kanMX6 ade6-M210 leu1-32 ura4-D18 | This study |
| KS2528 | h− alp4-3HA::kanMX alp16-13Myc::kanMX6 ade6-216 leu1-32 ura4-D18 | This study |
| KS2530 | h+ alp16-3HA::kanMX6 gfh1-13Myc::kanMX6 mod21Δ::hphMX ade6-M210 leu1-32 ura4-D18 | This study |
| KS2539 | h− alp4-3HA::kanMX alp16-13Myc::kanMX6 gfh1Δ::hphMX6 ade6-M216 leu1-32 ura4-D18 | This study |
| KS2541 | h+ alp4-3HA::kanMX alp16-13Myc::kanMX6 mod21Δ::hphMX6 ade6-M210 leu1-32 ura4-D18 | This study |
| KS2547 | h+ alp16-3HA::kanMX6 mod21-13Myc::kanMX6 gfh1Δ::hphMX6 ade6- leu1-32 ura4-D18 | This study |
| KS2549 | h+ alp4-3HA::kanMX alp16-13Myc::kanMX6 gfh1Δ::hphMX6 mod21Δ::hphMX6 ade6-M210 leu1-32 ura4-D18 | This study |
| KS2553 | h+ alp16-3HA::kanMX6 gfh1-13Myc::kanMX6 ade6-M210 leu1-32 ura4-D18 | This study |
| KS2557 | h− alp16-3HA::kanMX6 mod21-13Myc::kanMX6 ade6-M210 leu1-32 ura4-D18 | This study |
| KS2571 | h− alp16-GFP::kanMX6 ade6-M210 leu1-32 | This study |
| KS2861 | h− ars1::nmt81::GFP-atb2::LEU2 sad1-dsRed:LEU2 (sad1+) ade6-210 leu1-32 ura4-D18 | This study |
| KS2873 | h− ars1:nmt81:GFP-atb2:LEU2 sad1-dsRed:LEU2 (sad1+) mod22-1 ade6-210 leu1-32 ura4-D18 | This study |
| KS2876 | h− alp16Δ::natMX6 gfh1Δ::hphMX6 mod21Δ::kanMX6 ars1:nmt81:GFP-atb2:LEU2 ade6-210 leu1-32 ura4-D18 | This study |
| KS2881 | h− ars1:nmt81:GFP-atb2:LEU2 mod22-1 ade6-210 leu1-32 ura4-D18 | This study |
| KS2884 | h− kanMX6:nmt81:GFP-atb2 mod22-1 ade6-210 leu1-32 ura4-D18 | This study |
| KS2887 | h+ alp4-GFP:kanMX sad1-dsRed:LEU2 leu1-32 | This study |
| KS2893 | h+ gfh1-GFP:kanMX sad1-dsRed:LEU2 leu1-32 | This study |
| KS2903 | h+ mod21-GFP:kanMX sad1-dsRed:LEU2 leu1-32 | This study |
| KS2917 | h− gfh1Δ::hphMX6 ars1:nmt81:GFP-atb2:LEU2 sad1-dsRed:LEU2 (sad1+) ade6-210 leu1-32 ura4-D18 | This study |
| KS2918 | h− mod21Δ::kanMX6 ars1:nmt81:GFP-atb2:LEU2 sad1-dsRed:LEU2 (sad1+) ade6-210 leu1-32 ura4-D18 | This study |
| KS2919 | h− alp16Δ::natMX6 ars1:nmt81:GFP-atb2:LEU2 sad1-dsRed:LEU2 (sad1+) ade6-210 leu1-32 ura4-D18 | This study |
| KS2923 | h− alp16Δ::natMX6 gfh1Δ::hphMX6 mod21Δ::kanMX6 ars1:nmt81:GFP-atb2:LEU2 sad1-dsRed:LEU2 (sad1+) ade6-210 leu1-32 ura4-D18 | This study |
| KS2924 | h− gfh1Δ::hphMX6 mod22-1 ars1:nmt81:GFP-atb2:LEU2 sad1-dsRed:LEU2 (sad1+) ade6-210 leu1-32 ura4-D18 | This study |
| KS2925 | h− mod21Δ::kanMX6 mod22-1 ars1:nmt81:GFP-atb2:LEU2 sad1-dsRed:LEU2 (sad1+) ade6-210 leu1-32 ura4-D18 | This study |
| KS2930 | h− alp16Δ::natMX6 gfh1Δ::hphMX6 mod21Δ::kanMX6 mod22-1 ars1:nmt81:GFP-atb2:LEU2 sad1-dsRed:LEU2 (sad1+) ade6-210 leu1-32 ura4-D18 | This study |
| KS2946 | h− alp16Δ::natMX6 gfh1Δ::hphMX6 mod21Δ::kanMX6 kanMX6:nmt81:GFP-atb2 ade6-210 leu1-32 ura4-D18 | This study |
| KS2949 | h− alp16Δ::natMX6 ars1:nmt81:GFP-atb2:LEU2 ade6-210 leu1-32 ura4-D18 | This study |
| KS2952 | h− alp16Δ::natMX6 mod22-1 ars1:nmt81:GFP-atb2:LEU2 ade6-210 leu1-32 ura4-D18 | This study |
| KS2974 | h+ alp16-GFP:kanMX sad1-dsRed:LEU2 leu1-32 | This study |
| KS3002 | h− alp16Δ::natMX kanMX6:nmt81:GFP-atb2 ade6-210 leu1-32 ura4-D18 | This study |
| KS3006 | h− alp16Δ::natMX kanMX6:nmt81:GFP-atb2 mod22-1 ade6-210 leu1-32 ura4-D18 | This study |
| KS3123 | h− gfh1-3HA::kanMX6 mod22-1 ade6- leu1-32 ura4-D18 | This study |
| KS3126 | h− gfh1-13Myc::kanMX6 mod22-1 ade6- leu1-32 ura4-D18 | This study |
| KS3130 | h− gfh1-GFP::kanMX6 mod22-1 ade6- leu1-32 ura4-D18 | This study |
| KS3132 | h+ mod21-GFP::kanMX6 mod22-1 ade6- leu1-32 ura4-D18 | This study |
| KS3139 | h+ alp16-GFP::kanMX6 mod22-1 ade6- leu1-32 ura4-D18 | This study |
| KS3141 | h+ mod21-13Myc::kanMX6 mod22-1 ade6- leu1-32 ura4-D18 | This study |
| KS3145 | h− alp4-3HA::kanMX6 mod22-1 ade6- leu1-32 ura4-D18 | This study |
| KS3147 | h+ alp6-3HA::kanMX6 mod22-1 ade6- leu1-32 ura4-D18 | This study |
| KS3149 | h+ alp4-3HA::kanMX6 alp16-Myc::kanMX6 mod22-1 ade6- leu1-32 ura4-D18 | This study |
| KS3151 | h+ alp16-3HA::kanMX6 mod22-1 ade6- leu1-32 ura4-D18 | This study |
| KS3159 | h− alp16-13Myc::kanMX6 mod22-1 ade6- leu1-32 ura4-D18 | This study |
| KS3260 | h− alp4-GFP::kanMX6 mod22-1 ade6- leu1-32 ura4-D18 | This study |