Table 3.

Astral microtubules are not released from spindle poles in gfh1Δ, mod21Δ, and additional mutants

Strain	Relevant genotype	GFP-atb2p	Total cells observed	Total astral microtubule histories	Histories with length = 1 frame	Histories with length >1 frame	Histories with length >1 frame and astral MT release
KS1236	Wild type	Alone	6	18	11	7	0
KS1225	Wild type	With atb2+	7	27	5	22	0 (1?)a
KS1704	gfh1Δ	Alone	4	13	4	9	0
KS1708	gfh1Δ	With atb2+	4	22	9	13	0
KS1720	gfh1Δ mod22-1	Alone	9	25	6	19	0
KS1727	gfh1Δ mod22-1	With atb2+	7	12	1	11	1 (2?)
KS1696	mod21Δ	Alone	9	18	5	13	0 (1?)
KS1701	mod21Δ	With atb2+	11	46	5	41	1
KS1724	mod21Δ mod22-1	Alone	7	33	9	24	0
KS1733	mod21Δ mod22-1	With atb2+	10	42	9	33	1 (2?)
KS1812	alp16Δ gfh1Δ mod21Δ mod22-1	Alone	10	44	13	31	0
KS1817	alp16Δ gfh1Δ mod21Δ mod22-1	With atb2+	22	98	16	82	2–4b

Thirty-second frame intervals were used throughout for data acquisition and measurement. Further details can be found in Materials and Methods. Cells were scored from the time of spindle formation up to the beginning of formation of the PAA from the eMTOC.

^a (?) includes astral microtubules (MTs) that may have released, but only when eMTOC was already considerably active in forming the PAA, and thus cell cycle-dependent changes in microtubule behavior may already be occurring at the SPB.

^b In two of these instances, spindles had elongated all the way to cell tips and astral MTs were forced to bend; in the other two instances, two successive release events were observed in one individual cell, both from the same spindle pole.