Figure 2.

Sequence comparison of selected Sec4p homologues. Cn, C. neoformans Sav1p; Sp, S. pombe Ypt2p; Cl, C. lindemuthianum Clpt1p; An, Aspergillus niger SrgAp; Hs, Homo sapiens Rab8p; Sc, S. cerevisiae Sec4p. Residues that are identical in all sequences are shaded black; those conserved in four or five of six sequences are in gray. G1–G5 indicate conserved GTP binding domains, the vertical arrow indicates the conserved Val residue that was mutagenized to Asn in S. pombe (Craighead et al., 1993), and a conserved prenylation site at the C-terminus is marked with asterisks.